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Many resistant strains had been generated due to the intensive usage for the antibiotics either by human beings or veterinary affairs. Antimicrobial susceptibility testing for isolates obtained from patients’ samples revealed multidrug resistance profiles. Almost all isolated bacterial samples (nearly 90%) revealed high resistance. current study aimed for the investigation of the susceptibility rates of isolated bacterial species which are: E coli (60 samples), Klebsiella (27 samples), Salmonella typhi (1 sample) Serracia marcescens (4 samples), Enterobacter Cloacae (8 samples) and (Morganella morgani) in Al-Yarmouk hospital located in Baghdad (IRAQ). Clinical patient’s specimens were gotten from various biological sources followed by the biochemical testing to be diagnosed and identified correctly, then the use of VITEC-2 system software occured. Using the antibiotics of: Tricarcillin , clavulanic acid, piperacillin , tamzbacta, ceftazidime, cefepime, aztreonam, imipenem, meropenem, amikacin, gentamicin, tobramycinhis bacteria, ciprofloxacin, minocyclin I3, trimethoprim/ sulfamethoxazole; showed that there was a higher incidence of multidrug-resistance for all the tested bacterial strains; that E.coli showed 96% resistance against imipenem antibiotic making it the most ineffective drug against it; while Klebsiella bacteria showed 100% resistance against clavulanic acid and piperacillin antibiotics. When Serracia marcescens and Enterobacter cloacae showed resistance towards almost all the antibiotics used at this study. For Morganella morgani and Salmonella typhi, they showed resistance to some of the used antibiotics and were sensitive to the others. The detection of microorganisms at this high resistance to wide range of antibiotics put us in front of serious problem in present time. Thus, clinicians might benefit from these data to enhance the usage of antibiotics and follow moderate protocol to minimize the hazards.